GeneOracle

Gene Oracle's combination of our GeneIOS technology with high quality reagents and oligonucleotides, enables us to manufacture the genes and DNA sequences that you need for your research, quickly and reliably. Don't be limited by restriction sites and polylinkers, make the sequence that you require.

Cloning and Sub-cloning

Cloning and Sub-cloning

Gene Oracle offers free cloning for genes we synthesize into a standard cloning vector. Our vectors does not contain a multiple cloning site to minimize restriction sites, leaving most sites unique that have been included on your synthetic gene. This makes sub-cloning straight forward. For most applications our standard pGOV4 vector is most appropriate, instances where the synthesis product is similar in size to pGOV4, we recommend using pGOV5 or pGOV6.

Standard Vectors

Standard vectors are interchangeable and no extra fee will be charged for synthesis of standard synthetic genes.
All standard genes are subcloned into our standard cloning vector non-directionally. pGOV sequencing Forward & Reverse primers are used for sequence validation of all standard genes.

Vector Size (kb) Antibiotic resistance Sequence Map
pGOV4 3.3 Kan/Amp Download Download
pGOV5 2.5 Amp Download Download
pGOV6 2.5 Kan Download Download

For a listing of the standard cloning vectors we offer please contact our technical support team at .(JavaScript must be enabled to view this email address)

Custom Cloning

cDNA cloning - You can provide cDNA to be subcloned, we clone by traditional methods as well and next-gen cloning, Gibson or infusion type cloning.  All constructs are double strand sequence verified.

Subcloning

Allow us to help speed up your research by providing standard subcloning services. We use a combination of tranditonal methods and next-gen cloning approaches. Each construct will be double strand DNA sequence verified.

Custom Vector Construction:

Allow our cloning experts to evaluate and develop a cloning strategy or help design a vector for multi-step manipulation or for cloning and expression projects with current and subsequent cloning requirements.