Cloning and Sub-cloning
Gene Oracle offers free cloning for genes we synthesize into a standard cloning vector. Our vectors does not contain a multiple cloning site to minimize restriction sites, leaving most sites unique that have been included on your synthetic gene. This makes sub-cloning straight forward. For most applications our standard pGOV4 vector is most appropriate, instances where the synthesis product is similar in size to pGOV4, we recommend using pGOV5 or pGOV6.
Standard vectors are interchangeable and no extra fee will be charged for synthesis of standard synthetic genes.
All standard genes are subcloned into our standard cloning vector non-directionally. pGOV sequencing Forward & Reverse primers are used for sequence validation of all standard genes.
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cDNA cloning - You can provide cDNA to be subcloned, we clone by traditional methods as well and next-gen cloning, Gibson or infusion type cloning. All constructs are double strand sequence verified.
Allow us to help speed up your research by providing standard subcloning services. We use a combination of tranditonal methods and next-gen cloning approaches. Each construct will be double strand DNA sequence verified.
Custom Vector Construction:
Allow our cloning experts to evaluate and develop a cloning strategy or help design a vector for multi-step manipulation or for cloning and expression projects with current and subsequent cloning requirements.