Mutants & Mutagenesis
Explore sequence space or generate genetic diversity by creating mutants. Gene Oracle can generate simple point mutations in coding and non-coding sequences, or combine mutations using a combinatorial approach.
Mutants can be generated as part of an existing gene synthesis order or mutants alone, if not part of an existing order the template can be sent to Gene Oracle, and we will use as the wild type backbone. For templates that are sent, please provide the DNA sequence to the insert and the plasmid, in either text or genbank format.
For non-coding DNA sequences provide the backbone sequence for mutagenesis, and a list of the changes that are required, in the following format (current base-position-mutation) For coding sequences provide either the DNA sequence (specifically the reading frame), or the amino acid sequence and a list of the appropriate changes in the following format (current AA-position- new AA) (e.g. A100V). Fusions and fusion libraries can be made as part of an existing synthesis order or as individually. Some advantages of fusion proteins: Chimeras of fusion may be more stable Chimeras or fusions may improve solubility Fusions may be removed by including a sequence specific protease site, such as thrombin, enterokinase, or Xa