Gene Oracle offers free cloning for genes we synthesize into a standard cloning vector. Our vectors do not contain a multiple cloning site (MCS) in order to minimize restriction sites, leaving most sites unique that have been included on your synthetic gene. This makes subcloning straight forward. For most applications our standard pGOV4 vector is most appropriate, instances where the synthesis product is similar in size to pGOV4, we recommend using pGOV5 or pGOV6. Standard vectors are interchangeable and no extra fee will be charged. All standard genes are subcloned into our standard cloning vector nondirectionally. pGOV sequencing Forward & Reverse primers are used for sequence validation of all standard genes.
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